Antibody

id 106
Antibody name H4K20me3
Host organism Rabbit
Poly-/Monoclonal Polyclonal
Target H4K20me3
Peptide AKRHRK(me3)VLRDN
Reacts with human, broad range expected
Specificity Testing
H4K20me3, lot A2730P ChIP assay
ChIP assays were performed using human K562 cells and the Diagenode antibody against H4K2…
Chromatin Immunoprecipitation
Recommendation Recommended
Dilution 0.5 µg/ChIP
Chromatin Amount 100 000 cells
Chromatin Source K562 cells
Capture Method protein A coated magnetic beads
Protocols fixed chromatin
Protocol references diagenode.com/en/catalog/chromatin-function-74/chromatin-immunoprecipitation-75/manual-chip-kits-77/product/…
Comments This antibody was tested on 10e5 K562 cells using the Diagenode iDeal ChIP-seq kit (AB-001-0024)using 0.2 to 2 µg of antibody.
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h4-and-modified-h4-28/product/…
H4K20me3, lot A2730P ChIP-seq profile, chromosome…
ChIP-seq was performed with the antibody against H4K20me3 and the IP'd DNA was analysed o…
H4K20me3, lot A2730P ChIP-seq profime, ZNF510 pos…
ChIP-seq was performed with the antibody against H4K20me3 and the IP'd DNA was analysed o…
H4K20me3, lot A2730P ChIP-seq profile, chromosome…
ChIP-seq was performed with the antibody against H4K20me3 and the IP'd DNA was analysed o…
ChIP-sequencing
Recommendation Recommended
Dilution 0.5 µg/ChIP
Chromatin Amount 100 000 cells
Chromatin Source K562 cells
Capture Method protein A coated magnetic beads
Protocols fixed chromatin
Protocol references diagenode.com/en/catalog/chromatin-function-74/chromatin-immunoprecipitation-75/manual-chip-kits-77/product/…
Comments ChIP was performed on sheared chromatin from 100 000 K562 cells using 0.5 µg of the antibody against H4K20me3 with the "iDeal ChIP-seq" kit (Diagenode, cat. No. AB-001-0024).
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h4-and-modified-h4-28/product/…
H4K20me3, lot A2730P dot blot analysis
To test the cross reactivity of the Diagenode antibody against H4K20me3, a Dot Blot analy…
Dot blot
Recommendation Recommended
Dilution 1/20000
Blocker TBS-T containing 5% skimmed milk
Incubation 1 hour at room temperature
Secondary Ab ECL peroxidase labelled anti-rabbit antibody (GE Healthcare)
Comments Dot blot was performed for 1 hour at room temperature in TBS-T containing 5% skimmed milk. An ECL peroxidase labelled anti-rabbit antibody (GE Healthcare) was used as secondary antibody
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h4-and-modified-h4-28/product/…
H4K20me3, lot A2730P, ELISA result
To determine the titer of the antibody, an ELISA was performed using a serial dilution of…
Enzyme Linked Immunosorbent Assay
Recommendation Recommended
Dilution dilution curve
Blocker PBS-T containing 3% BSA
Incubation overnight, 4°C
Secondary Ab Goat Anti Rabbit HRP conjugated (Sigma)
Comments ELISA was performed to determine the titer of the antibody. The titer was estimated to be 1/21700
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h4-and-modified-h4-28/product/…
H4K20me3, lot A2730P IF assay
HeLa cells were stained with the Diagenode antibody against H4K20me3 and with DAPI. Cells…
Immunofluorescence
Recommendation Recommended
Dilution 1/500
Fixation methanol
Permeabilization 0.5% Triton X-100 in PBS, 15 min RT
Blocker PBS containing 5% normal goat serum, 1% BSA, 0.1% Triton X-100
Incubation 4°C overnight
Secondary Ab Goat Anti-Rabbit Alexa 488, Invitrogen (A11008)
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h4-and-modified-h4-28/product/…
H4K20me3, lot A2730P WB assay
Western blot was performed on whole cell (25 µg, lane 1) and histone extracts (15 µg, lan…
Western blot
Recommendation Recommended
Dilution 1/1000
Blocker TBS-T containing 5% skimmed milk
Incubation overnight, 4°C
Secondary Ab ECL peroxidase labelled anti-rabbit antibody (GE Healthcare)
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h4-and-modified-h4-28/product/…
H4K20me3, lot A2730P peptide array
The specificity of the antibody was further demonstrated by peptide array analyses on an …
H4K20me3, lot A2730P, ranked list of peptides
Normalized intensities of the different peptides present on the array.
peptide array
Recommendation Recommended
Comments Peptide array was performed for 1 hour at room temperature in TBS-T containing 5% skimmed milk. The antibody was used at a dilution of 1/10000.
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h4-and-modified-h4-28/product/…
Supplier
Company: Diagenode
Lot no: A2730P
Catalog no: C15410207
Website: http://www.diagenode.com
Contact email: info@diagenode.com
This data is online since 2013-04-09 15:54
Last update 2013-04-25 12:35
provided by Jan Hendrickx