Antibody

id 107
Antibody name H3K9/14ac
Host organism Rabbit
Poly-/Monoclonal Polyclonal
Target H3K9/14ac
Peptide TARK(ac)STGGK(ac)APR
Reacts with human, broad range expected
Specificity Testing
H3K9/14ac, lot A1756D ChIP assay on 10e6 HeLa cel…
ChIP assays were performed using human HeLa cells and the Diagenode antibody against H3K9…
H3K9/14ac, lot A1756D ChIP assay on 10e6 HeLa cel…
ChIP was performed using 1 µg of the Diagenode antibody against H3K9/14ac on 100 000 K562…
Chromatin Immunoprecipitation
Recommendation Recommended
Dilution 1 µg/ChIP
Chromatin Amount 1 000 000 - 100 000 cells
Chromatin Source K562, HeLa cells
Capture Method protein A coated magnetic beads
Protocols fixed chromatin
Protocol references diagenode.com/en/catalog/chromatin-function-74/chromatin-immunoprecipitation-75/manual-chip-kits-77/product/… and diagenode.com/en/catalog/chromatin-function-74/chromatin-immunoprecipitation-75/…-
Comments ChIP was performed with the Diagenode antibody against H3K9/14ac on sheared chromatin from 1 000 000 HeLa using the "Auto Histone ChIP-seq" kit (Diagenode, cat. No. AB-Auto02-A100) and from 100 000 K562 cells with the "iDeal ChIP-seq" kit (Diagenode, cat. No. AB-001-0024)
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h3-and-modified-h3-27/product/…
H3K9/14ac, lot A1756D ChIP-seq profile, complete …
ChIP-seq was performed with the antibody against H3K9/14ac and the IP'd DNA was analysed …
H3K9/14ac, lot A1756D ChIP-seq profile, X-chromos…
ChIP-seq was performed with the antibody against H3K9/14ac and the IP'd DNA was analysed …
H3K9/14ac, lot A1756D ChIP-seq profile, GAPDH pos…
ChIP-seq was performed with the antibody against H3K9/14ac and the IP'd DNA was analysed …
H3K9/14ac, lot A1756D ChIP-seq profile, EIF4A2 po…
ChIP-seq was performed with the antibody against H3K9/14ac and the IP'd DNA was analysed …
ChIP-sequencing
Recommendation Recommended
Dilution 1 µg/ChIP
Chromatin Amount 100 000 cells
Chromatin Source K562 cells
Capture Method protein A coated magnetic beads
Protocols fixed chromatin
Protocol references diagenode.com/en/catalog/chromatin-function-74/chromatin-immunoprecipitation-75/manual-chip-kits-77/product/…
Comments ChIP was performed on sheared chromatin from 100 000 K562 cells using 1 µg of the antibody against H3K9/14ac with the "iDeal ChIP-seq" kit (Diagenode, cat. No. AB-001-0024)
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h3-and-modified-h3-27/product/…
H3K9/14ac, lot A1756D dot blot analysis
To test the cross reactivity of the Diagenode antibody against H3K9/14ac, a Dot Blot anal…
Dot blot
Recommendation Recommended
Dilution 1/20000
Blocker TBS-T containing 5% skimmed milk
Incubation 1 hour at room temperature
Secondary Ab ECL peroxidase labelled anti-rabbit antibody (GE Healthcare)
Comments Dot blot was performed for 1 hour at room temperature in TBS-T containing 5% skimmed milk. An ECL peroxidase labelled anti-rabbit antibody (GE Healthcare) was used as secondary antibody
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h3-and-modified-h3-27/product/…
H3K9/14ac, lot A1756D ELISA result
To determine the titer of the antibody, an ELISA was performed using a serial dilution of…
Enzyme Linked Immunosorbent Assay
Recommendation Recommended
Dilution dilution curve
Blocker PBS-T containing 3% BSA
Incubation overnight, 4°C
Secondary Ab Goat Anti Rabbit HRP conjugated (Sigma)
Comments ELISA was performed to determine the titer of the antibody. The titer was estimated to be 1/4000
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h3-and-modified-h3-27/product/…
H3K9/14ac, lot A1756D IF assay
HeLa cells were stained with the Diagenode antibody against H3K9/14ac and with DAPI. Cell…
Immunofluorescence
Recommendation Recommended
Dilution 1/200
Fixation 4% formaldehyde, 10’ at RT
Permeabilization 0.5% Triton X-100 in PBS, 15 min RT
Blocker PBS containing 5% normal goat serum, 1% BSA, 0.1% Triton X-100
Incubation 4°C overnight
Secondary Ab Goat Anti-Rabbit Alexa 488, Invitrogen (A11008)
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h3-and-modified-h3-27/product/…
H3K9/14ac, lot A1756D WB assay
Western blot was performed on whole cell (25 µg, lane 1) and histone extracts (15 µg, lan…
Western blot
Recommendation Recommended
Dilution 1/500
Blocker TBS-T containing 5% skimmed milk
Incubation overnight, 4°C
Secondary Ab ECL peroxidase labelled anti-rabbit antibody (GE Healthcare)
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h3-and-modified-h3-27/product/…
H3K9/14ac, lot A1756D peptide array
The specificity of the antibody was further demonstrated by peptide array analyses on an …
H3K9/14ac, lot A1756D peptide array, ranked list …
Normalized intensities of the different peptides present on the array.
peptide array
Recommendation Recommended
Comments Peptide array was performed for 1 hour at room temperature in TBS-T containing 5% skimmed milk. The antibody was used at a dilution of 1/2000.
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h3-and-modified-h3-27/product/…
Supplier
Company: Diagenode
Lot no: A1756D
Catalog no: C15410200
Website: http://www.diagenode.com
Contact email: info@diagenode.com
This data is online since 2013-04-19 14:10
Last update 2013-04-25 12:43
provided by Jan Hendrickx