Antibody

id 108
Antibody name H2A.Zac
Host organism Rabbit
Poly-/Monoclonal Polyclonal
Target H2A.ZK4,7,11ac
Peptide GGK(ac)AGK(ac)DSGK(ac)AK
Reacts with human, broad range expected
Specificity Testing
H2A.Zac, lot A1738P ChIP assay on 10e6 HeLa cells
ChIP assays were performed using human HeLa cells with the Diagenode antibody against H2…
H2A.Zac, lot A1738P ChIP assay on 10e5 K562 cells
ChIP assays were performed on human K562 cells with the Diagenode antibody against H2A.Za…
Chromatin Immunoprecipitation
Recommendation Recommended
Dilution 1 µg/ChIP
Chromatin Amount 1 000 000 - 100 000 cells
Chromatin Source K562, HeLa cells
Capture Method protein A coated magnetic beads
Protocols fixed chromatin
Protocol references diagenode.com/en/catalog/chromatin-function-74/chromatin-immunoprecipitation-75/automated-chip-kits-76/product/… and diagenode.com/p/…
Comments This antibody was tested on 10e6 HeLa cells with the Diagenode AutoHistone ChIP-seq kit (AB-Auto02-A100) using 1 to 10 µg of antibody, and on 10e5 K562 cells with the Diagenode iDeal ChIP-seq kit (AB-001-0024)using 0.2 to 2 µg of antibody.
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h2-and-modified-h2-42/product/…
H2A.Zac, lot A1738P ChIP-seq profile, X-chromosome
ChIP was performed on sheared chromatin from 100,000 K562 cells using 1 µg the Diagenode …
H2A.Zac, lot A1738P ChIP-seq profile, X-chromosom…
ChIP was performed on sheared chromatin from 100,000 K562 cells using 1 µg the Diagenode …
H2A.Zac, lot A1738P ChIP-seq profile, EIF4A2 posi…
ChIP was performed on sheared chromatin from 100,000 K562 cells using 1 µg the Diagenode …
H2A.Zac, lot A1738P ChIP-seq profile, CCT5 positi…
ChIP was performed on sheared chromatin from 100,000 K562 cells using 1 µg the Diagenode …
ChIP-sequencing
Recommendation Recommended
Dilution 1 µg/ChIP
Chromatin Amount 100 000 cells
Chromatin Source K562 cells
Capture Method protein A coated magnetic beads
Protocols fixed chromatin
Protocol references diagenode.com/en/catalog/chromatin-function-74/chromatin-immunoprecipitation-75/manual-chip-kits-77/product/…
Comments ChIP was performed on sheared chromatin from 100 000 K562 cells using 0.5 µg of the antibody against H4K20me3 with the "iDeal ChIP-seq" kit (Diagenode, cat. No. AB-001-0024).
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h2-and-modified-h2-42/product/…
H2AZac, lot A1738P dot blot analysis
To test the cross reactivity of the Diagenode antibody against H2A.Zac (cat. No. C1541020…
Dot blot
Recommendation Recommended
Dilution 1/20000
Blocker TBS-T containing 5% skimmed milk
Incubation 1 hour at room temperature
Secondary Ab ECL peroxidase labelled anti-rabbit antibody (GE Healthcare)
Comments Dot blot was performed for 1 hour at room temperature in TBS-T containing 5% skimmed milk. An ECL peroxidase labelled anti-rabbit antibody (GE Healthcare) was used as secondary antibody
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h2-and-modified-h2-42/product/…
H2A.Zac, lot A1738P, ELISA result
To determine the titer of the antibody, an ELISA was performed using a serial dilution of…
Enzyme Linked Immunosorbent Assay
Recommendation Recommended
Dilution dilution curve
Blocker PBS-T containing 3% BSA
Incubation overnight, 4°C
Secondary Ab Goat Anti Rabbit HRP conjugated (Sigma)
Comments ELISA was performed to determine the titer of the antibody. The titer was estimated to be 1/56600
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h2-and-modified-h2-42/product/…
H2A.Zac, lot A1738P IF assay
HeLa cells were stained with the Diagenode antibody against H2A.Zac and with DAPI. Cells …
Immunofluorescence
Recommendation Recommended
Dilution 1/500
Fixation 4% formaldehyde, 10’ at RT
Permeabilization 0.5% Triton X-100 in PBS, 15 min RT
Blocker PBS containing 5% normal goat serum, 1% BSA, 0.1% Triton X-100
Incubation 4°C overnight
Secondary Ab Goat Anti-Rabbit Alexa 488, Invitrogen (A11008)
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h2-and-modified-h2-42/product/…
H2A.Zac, lot A1738P, WB result
Western blot was performed on whole cell extracts (25 µg, lane 1) from HeLa cells, and on…
Western blot
Recommendation Recommended
Dilution 1/1000
Blocker TBS-T containing 5% skimmed milk
Incubation overnight, 4°C
Secondary Ab ECL peroxidase labelled anti-rabbit antibody (GE Healthcare)
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h2-and-modified-h2-42/product/…
Supplier
Company: Diagenode
Lot no: A1738P
Catalog no: C15410202
Website: http://www.diagenode.com
Contact email: info@diagenode.com
This data is online since 2013-04-25 11:50
Last update 2016-12-21 09:27
provided by Jan Hendrickx