Antibody

id 109
Antibody name H2A.Z
Host organism Rabbit
Poly-/Monoclonal Polyclonal
Target H2A.Z
Peptide [no data entered]
Reacts with human
Specificity Testing
H2A.Z, lot A2039P ChIP assay on 10e6 HeLa cells
ChIP assays were performed on human HeLa cells with the Diagenode antibody against H2A.Z)…
H2A.Z, lot A2039P ChIP assay on 10e5 K562 cells
ChIP assays were performed on human K562 cells with the Diagenode antibody against H2A.Z.…
Chromatin Immunoprecipitation
Recommendation Recommended
Dilution 0.5 - 1 µg/ChIP
Chromatin Amount 1 000 000 - 100 000 cells
Chromatin Source K562, HeLa cells
Capture Method protein A coated magnetic beads
Protocols fixed chromatin
Protocol references diagenode.com/en/catalog/chromatin-function-74/chromatin-immunoprecipitation-75/automated-chip-kits-76/product/… and diagenode.com/en/catalog/chromatin-function-74/chromatin-immunoprecipitation-75/…-
Comments This antibody was tested on 10e6 HeLa cells with the Diagenode AutoHistone ChIP-seq kit (AB-Auto02-A100) using 1 to 10 µg of antibody, and on 10e5 K562 cells with the Diagenode iDeal ChIP-seq kit (AB-001-0024)using 0.2 to 2 µg of antibody.
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h2-and-modified-h2-42/product/…
H2A.Z, lot A2039P ChIP-seq profile, EIF2S3 positi…
ChIP was performed on sheared chromatin from 100,000 K562 cells using 0.5 µg the Diagenod…
H2A.Z, lot A2039P ChIP-seq profile, chromosome 12…
ChIP was performed on sheared chromatin from 100,000 K562 cells using 0.5 µg the Diagenod…
H2A.Z, lot A2039P ChIP-seq profile, X-chromosome …
ChIP was performed on sheared chromatin from 100,000 K562 cells using 0.5 µg the Diagenod…
H2A.Z, lot A2039P ChIP-seq profile, c-fos positiv…
ChIP was performed on sheared chromatin from 100,000 K562 cells using 0.5 µg the Diagenod…
ChIP-sequencing
Recommendation Recommended
Dilution 0.5 µg/ChIP
Chromatin Amount 100 000 cells
Chromatin Source K562 cells
Capture Method protein A coated magnetic beads
Protocols fixed chromatin
Protocol references diagenode.com/en/catalog/chromatin-function-74/chromatin-immunoprecipitation-75/manual-chip-kits-77/product/…
Comments ChIP was performed on sheared chromatin from 100 000 K562 cells using 0.5 µg of the antibody against H4K20me3 with the "iDeal ChIP-seq" kit (Diagenode, cat. No. AB-001-0024)
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h2-and-modified-h2-42/product/…
H2A.Z, lot A2039P, ELISA result
To determine the titer of the antibody, an ELISA was performed using a serial dilution of…
Enzyme Linked Immunosorbent Assay
Recommendation Recommended
Dilution dilution curve
Blocker PBS-T containing 3% BSA
Incubation overnight, 4°C
Secondary Ab Goat Anti Rabbit HRP conjugated (Sigma)
Comments ELISA was performed to determine the titer of the antibody. The titer was estimated to be 1/87500
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h2-and-modified-h2-42/product/…
H2A.Z, lot A2039P IF assay
HeLa cells were stained with the Diagenode antibody against H2A.Z and with DAPI. Cells we…
Immunofluorescence
Recommendation Recommended
Dilution 1/500
Fixation 4% formaldehyde, 10’ at RT
Permeabilization 0.5% Triton X-100 in PBS, 15 min RT
Blocker PBS containing 5% normal goat serum, 1% BSA, 0.1% Triton X-100
Incubation 4°C overnight
Secondary Ab Goat Anti-Rabbit Alexa 488, Invitrogen (A11008)
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h2-and-modified-h2-42/product/…
H2A.Z, lot A2039P, WB result
Western blot was performed on whole cell (25 µg, lane 1) and histone extracts (15 µg, lan…
Western blot
Recommendation Recommended
Dilution 1/1000
Blocker TBS-T containing 5% skimmed milk
Incubation overnight, 4°C
Secondary Ab ECL peroxidase labelled anti-rabbit antibody (GE Healthcare)
Link to antibody source diagenode.com/en/catalog/antibodies-3/histone-h2-and-modified-h2-42/product/…
Supplier
Company: Diagenode
Lot no: A2039P
Catalog no: C15410201
Website: http://www.diagenode.com
Contact email: info@diagenode.com
This data is online since 2013-04-25 13:10
Last update 2013-06-06 12:50
provided by Jan Hendrickx