Antibody

id 111
Antibody name Pol 2
Host organism Mouse
Poly-/Monoclonal Monoclonal
Target Pol 2
Peptide confidential
Reacts with human, mouse, broad range expected
Specificity Testing
Pol , lot 001-11 ChIP results
ChIP assays were performed using human HeLa cells, the Diagenode monoclonal antibody agai…
Chromatin Immunoprecipitation
Recommendation Recommended
Dilution 1 µg/ChIP
Chromatin Amount 1 000 000 cells
Chromatin Source HeLa cells
Capture Method protein A/G coated magnetic beads
Protocols fixed chromatin
Protocol references iDeal ChIP-seq kit (diagenode.com/en/catalog/chromatin-function-74/chromatin-immunoprecipitation-75/manual-chip-kits-77/product/…)
Link to antibody source diagenode.com/en/catalog/antibodies-3/transcription-transcription-factor-32/product/…
Pol 2, lot 001-11, ChIP-seq profile along the X c…
ChIP was performed on sheared chromatin from 1 million HeLaS3 cells using 1 µg of the Dia…
Pol 2, lot 001-11, ChIP-seq profile along a 400 k…
ChIP was performed on sheared chromatin from 1 million HeLaS3 cells using 1 µg of the Dia…
Pol 2, lot 001-11, ChIP-seq signal surrounding th…
ChIP was performed on sheared chromatin from 1 million HeLaS3 cells using 1 µg of the Dia…
Pol 2, lot 001-11, ChIP-seq signal surrounding th…
ChIP was performed on sheared chromatin from 1 million HeLaS3 cells using 1 µg of the Dia…
ChIP-sequencing
Recommendation Recommended
Dilution 1 µg/ChIP
Chromatin Amount 1 000 000 cells
Chromatin Source HeLa cells
Capture Method protein A coated magnetic beads
Protocols fixed chromatin
Protocol references iDeal ChIP-seq kit (diagenode.com/en/catalog/chromatin-function-74/chromatin-immunoprecipitation-75/manual-chip-kits-77/product/…)
Link to antibody source diagenode.com/en/catalog/antibodies-3/transcription-transcription-factor-32/product/…
Pol 2, lot 001-11, ELISA specificity test
To test the specificity an ELISA was performed using a serial dilution of the Diagenode m…
Enzyme Linked Immunosorbent Assay
Recommendation Recommended
Dilution 1:3,000
Blocker PBS-T containing 3% BSA
Incubation overnight, 4°C
Comments ELISA was performed to determine the specificty of the antibody
Link to antibody source diagenode.com/en/catalog/antibodies-3/transcription-transcription-factor-32/product/…
Pol 2, lot 001-11, IF assay
HeLa cells were stained with the Diagenode antibody against Pol II (cat. No. C15200004) a…
Immunofluorescence
Recommendation Recommended
Dilution 1:500
Fixation methanol
Permeabilization 0.5% Triton X-100 in PBS, 15 min RT
Blocker PBS containing 5% normal goat serum, 1% BSA, 0.1% Triton X-100
Incubation 4°C overnight
Secondary Ab Alrxa Fluor 594 Goat anti-mouse, Life technologies A11032
Link to antibody source diagenode.com/en/catalog/antibodies-3/transcription-transcription-factor-32/product/…
Pol 2, lot 001-11, WB assay
Nuclear extracts (25 µg) from HeLa cells were analysed by Western blot using the Diagenod…
Western blot
Recommendation Recommended
Dilution 1:1,000
Blocker TBS-T containing 5% skimmed milk
Incubation 4°C overnight
Secondary Ab ECL peroxidase labelled anti-mouse, Sigma A 9044-2ML antibody
Link to antibody source diagenode.com/en/catalog/antibodies-3/transcription-transcription-factor-32/product/…
Supplier
Company: Diagenode
Lot no: 001-11
Catalog no: C1520004
Website: http://www.diagenode.com
Contact email: info@diagenode.com
This data is online since 2014-11-17 16:43
Last update 2014-11-18 14:00
provided by Jan Hendrickx