Antibody

id 112
Antibody name H3K79me3
Host organism Rabbit
Poly-/Monoclonal Polyclonal
Target H3K79me3
Peptide EIAQDFKme3TDLRF
Reacts with human, mouse, broad range expected
Specificity Testing
H3K79me3, lot A2014P, ChIP results
ChIP assays were performed using human K562 cells, the Diagenode antibody against H3K79me…
Chromatin Immunoprecipitation
Recommendation Recommended
Dilution 1 µg/ChIP
Chromatin Amount 100 000 cells
Chromatin Source K562
Capture Method protein A coated magnetic beads
Protocols fixed chromatin
Protocol references iDeal ChIP-seq kit (diagenode.com/en/catalog/chromatin-function-74/chromatin-immunoprecipitation-75/manual-chip-kits-77/product/…)
Link to antibody source diagenode.com
H3K79me3, lot A2014, ChIP-seq results
ChIP was performed on sheared chromatin from 100,000 K562 cells with the
H3K79me3, lot A2014P, ChIP-seq results
ChIP was performed on sheared chromatin from 100,000 K562 cells with the
H3K79me3, lot A2014P, ChIP-seq results
ChIP was performed on sheared chromatin from 100,000 K562 cells with the
H3K79me3, lot A2014P, ChIP-seq results
ChIP was performed on sheared chromatin from 100,000 K562 cells with the
ChIP-sequencing
Recommendation Recommended
Dilution 1 µg/ChIP
Chromatin Amount 100 000 cells
Chromatin Source K562
Capture Method protein A coated magnetic beads
Protocols fixed chromatin
Protocol references iDeal ChIP-seq kit (diagenode.com/en/catalog/chromatin-function-74/chromatin-immunoprecipitation-75/manual-chip-kits-77/product/…)
Link to antibody source diagenode.com
H3K79me3, A2014P, dot blot analysis
To test the cross reactivity of the Diagenode antibody against H3K79me3 a Dot Blot analys…
Dot blot
Recommendation Recommended
Dilution 1:20,000
Blocker TBS-T containing 5% skimmed milk
Incubation 1 hour at room temperature
Secondary Ab ECL peroxidase labelled anti-rabbit antibody (GE Healthcare)
Link to antibody source diagenode.com
H3K79me3, lot A2014P, ELISA assay
To determine the titer of the antibody, an ELISA was performed using a serial dilution of…
Enzyme Linked Immunosorbent Assay
Recommendation Recommended
Dilution dilution curve
Blocker PBS-T containing 3% BSA
Incubation overnight, 4°C
Secondary Ab Goat Anti Rabbit HRP conjugated (Sigma)
Link to antibody source diagenode.com
H3K79me3, lot A2014P, IF results
HeLa cells were stained with the Diagenode antibody against H3K79me3 and with DAPI. Cells…
Immunofluorescence
Recommendation Recommended
Dilution 1:500
Fixation methanol
Permeabilization 0.5% Triton X-100 in PBS, 15 min RT
Blocker PBS containing 5% normal goat serum, 1% BSA, 0.1% Triton X-100
Incubation 4°C overnight
Secondary Ab Goat Anti-Rabbit Alexa 488, Invitrogen (A11008)
Link to antibody source diagenode.com/
H3K79me3, lot A2014P, WB results
Western blot was performed on whole cell (25 µg, lane 1) and histone extracts (15 µg, lan…
Western blot
Recommendation Recommended
Dilution 1:500
Blocker TBS-T containing 5% skimmed milk
Incubation 4°C overnight
Secondary Ab ECL peroxidase labelled anti-rabbit antibody (GE Healthcare)
Link to antibody source diagenode.com/
Supplier
Company: Diagenode
Lot no: A2014P
Catalog no: NA
Website: http://www.diagenode.com
Contact email: info@diagenode.com
This data is online since 2014-11-20 10:46
Last update 2014-11-20 11:26
provided by Jan Hendrickx