H3K4me3, lot A5051-001P, IF results
Human osteosarcoma (U2OS) cells were stained with the H3K4me3 antibody(lot A5051-001P) and with DAPI. Cells were fixed with 4% formaldehyde for 20’ and blocked with PBS/TX-100 containing 5% normal goat serum. The cells
were immunofluorescently labeled with the H3K4me3 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa568 or with DAPI (right), which specifically labels DNA.