H3K27ac, lot A1723-0041D, dot blot analysis

To test the cross reactivity of the Diagenode antibody against H3K27ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K27. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The figure shows a high specificity of the antibody for the modification of interest.
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