H3K27me3, lot A1811-001P IF assay
NIH3T3 cells were stained with the H3K27me3 antibody(lot A1811-001P) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS containing 5% normal goat serum, 1% BSA and 0.1% Triton X-100. The cells were immunofluorescently labeled
with the H3K27me3 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.