H3K27ac, lot A1723-0041D IF assay
HeLa cells were stained with the H3K27ac antibody(lot A1723-0041D) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS containing 5% normal goat serum, 1% BSA and 0.1% Triton X-100. The cells were immunofluorescently labeled
with the H3K36me3 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.