Pol 2, lot 001-11, ChIP-seq profile along the X chromosome
ChIP was performed on sheared chromatin from 1 million HeLaS3 cells using 1 µg of the Diagenode antibody against Pol II (cat. No. C15200004). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation
and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the peak distribution along the complete sequence of the X-chromosome.