Pol 2, lot 001-11, ELISA specificity test

To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against Pol II (cat. No. C15200004). The wells were coated with peptides containing the unmodified C-terminal repeat sequence as well as different phosphorylated peptides. The figure shows that the antibody recognizes the unphosphorylated Pol II as well as most phosphorylated forms.
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