Pol 2, lot 001-11, ELISA specificity test
To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against Pol II (cat. No. C15200004). The wells were coated with peptides containing the unmodified C-terminal repeat sequence as well as different phosphorylated peptides. The figure shows that the antibody recognizes the unphosphorylated Pol II as well as most phosphorylated forms.