H3K4me1, lot A1863-001P dot blot analysis

A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H3K4me1 with peptides containing other modifications or unmodified sequences of histone H3. One hundred to
0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The figure shows a high specificity of the antibody for the modification of interest.
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