H3K27me3, lot A1811-001P ChIP assay
ChIP assays were performed using human K562 cells, the Diagenode antibody against H3K27me3 and optimized PCR primer pairs for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. AB-001-0024), using sheared chromatin from 100 000 cells. A titration consisting of 0.1, 0.5, 1 and 2 µg of antibody per ChIP experiment was analyzed. IgG (1 µg/IP) was
used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as negative controls, and TSH2B and MYT1, used as positive controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).