H3K9me3, lot A1671-001P ChIP assay, 10e6 HeLa cells

ChIP assays were performed using human HeLa cells, the Diagenode antibody against H3K9me3 and optimized PCR primer pairs for qPCR. ChIP was performed with the "Auto Histone ChIP-seq" kit (cat. No. AB-Auto02-A100), using sheared chromatin from 1 million cells. A titration consisting of 0.2, 0.5, 1, 2 and 5 µg of antibody per ChIP experiment
was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and c-fos, used as negative controls, and for ZNF510 and the Sat2 satellite repeat, used as positive controls. The figure shows the recovery, expressed as a % of input
Go back to antibody H3K9me3

Download image in full resolution